Rhodopsin and Halobacterium, Complete Papers 2016-02-07

Rhodopsin and Halobacterium, Complete Papers

  1. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    Mustafa Umut Sarac submitted a new resource:

    Rhodopsin and Halobacterium, Complete Papers - Rhodopsin and Halobacterium, Complete Papers

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  2. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    Biosynthesis of Rhodopsin from Halobacteria

    STEP 2

    First post was about halobacteria growing.


    This paper relates to biosynthesis of rhodopsin from halobacteria.




    Biosynthesis of the Purple Membrane of Halobacteria




    By Manfred Sumper, Heribert Reitmeier, and Dieter Oesterhelt[*j
    Dedicated to Professor Feodor Ljinen on the occasion of his 65th birthday
    Halobacteria are extremely specialized organisms. They live exclusively in saturated solutions
    of common salt. The cell membrane of these bacteria exhibit insular regions which can be
    isolated by membrane fractionation. These regions consist of a lipid matrix containing bateriorhodopsin
    molecules in a hexagonal crystalline arrangement. Bacteriorhodopsin is a deep purple
    retinal-protein complex (“purple membrane”). The purple membrane functions as a light energy
    converter. --How can such a differentiated membrane region arise? In uiuo studies on the
    biosynthesis of the purple membrane showed another cell membrane fraction, the so-called
    brown membrane, to be a biosynthetic precursor. Bacterioopsin (the retinal-free protein) is
    initially incorporated into the brown membrane and can only form the purple membrane
    by crystallization in an energy-dependent reaction after prior reaction with retinal. This reaction
    is reversible. Removal of the retinal by formation of retinal oxime causes the purple membrane
    regions to disappear. Reconstitution of the bacteriorhodopsin by addition of retinal regenerates
    the purple membrane.​
     

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  3. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    Making film out of rhodopsin

    STEP 3

    You bought bacteria,grew it, synthesized it , now its time to make a film with your rhodopsin.



    Bacteriorhodopsin oriented in polyvinyl alcohol films as an erasable optical storage medium​




    Zhongping Chen, Aaron Lewis, Hiroyuki Takei, and Isaiah Nebenzahl
    Films of oriented bacteriorhodopsin have been formed in polyvinyl alcohol with excellent optical quality.
    Images with high contrast have been impressed and erased on these films. Second-harmonic microscopy has
    been used to read the image on a bacteriorhodopsin-polyvinyl alcohol film without erasure. The potential of
    these films for molecular information storage and computation is discussed.
    Keywords: Erasable optical storage, bacteriorhodopsin, biomolecular electronic devices​
     

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  4. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    Making 3D Optical Storage and knowing rewrite characteristics of RD

    STEP 4

    You completed the film and want to learn all about writing, erasing, rewriting characteristics of rhodopsin. This paper is a must and in 4 parts.

    PART 1


    AFRL-IF-RS-TR-2002-319
    Final Technical Report
    January 2003
    PROTOTYPE PROTEIN-BASED THREEDIMENSIONAL MEMORY
    Syracuse University
     

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  5. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    PART 2

    PART 2

    AFRL-IF-RS-TR-2002-319
    Final Technical Report
    January 2003
    PROTOTYPE PROTEIN-BASED THREE DIMENSIONAL MEMORY
    Syracuse University
     

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  6. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    PART 3

    PART 3
     

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  7. Mustafa Umut Sarac

    Mustafa Umut Sarac Member

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    PART 4

    PART 4
     

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  8. michael_r

    michael_r Subscriber

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    As this is not analog, but rather biological, it should be posted on APUG's sister site, BPUG.
     
  9. AgX

    AgX Member

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    I regard bio-sensors as analogue.
     
  10. BobCrowley

    BobCrowley Subscriber

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    I think this is genius. The eye uses rhodopsin (which means purple) and a chain of chemical events excite the neurons. Here it can be made into a biofilm and potentially be used as a photographic recording system of incredible sensitivity and resolution! It most certainly falls into the so-called analogue category to me.
     
  11. Rick A

    Rick A Subscriber

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    +1
     
  12. stormpetrel

    stormpetrel Member

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    Very interesting indeed! I will read all the papers carefully when I will have a moment.
     
  13. Truzi

    Truzi Subscriber

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    I get your joke, but... gelatin is/was biological, so would we need finer granularity than merely bio-PUG.