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  1. #1
    Mustafa Umut Sarac's Avatar
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    Picasso Rewritable Halobacterium Salinarum Emulsion

    I read picasso inspired from a broken camera and lens to make his one of the most important drawings of his lifetime. He was using a camera and lens broken and take parallel distortion images.

    BUT one of the most interesting thing , his final image being taken from his camera lens fungus after 40 years of that camera being found.

    One museum expert found fungus on the lens and fungus being scanned and his last known photograph developed. Its about the bacteriorhodopsin found in the fungus . That protein is in our eyes and saline waters makes waters colorful.

    Halobacterium salinarum is the responsible of generation about above protein , it loves salt , 37 celcius and stirring.

    If you mix rhodopsin in to plastic and enlarge an hologram , it records it for 1/10 second of time and than forgets. If you shine a photograph on to it and than in short time, if you shine a red laser on to the photograph , rhodopsin swithes to q state and never forgets again. If you shine a blue laser , it deletes the information.

    10 million times rewritable. And millions times more detail than dvd.

    If you prepare 10 plates for ultra large format , it lowers the costs hundreds times per year.

    This article is a start point , highschool children makes their big hologram plates with growing that protein and put in to plastic media.

    Once you accomplish a big film with it , it expands large and ultra large format in to the hands of many.

    Here is a recipe.

    Halobacterium salinarum Media
    Materials
    ● 250 g NaCl
    ● 20 g MgSO4
    ● 2 g KCl
    ● 3 g NaH2C6H5O7 (Sodium Citrate)
    ● 10 g Oxoid Peptone
    ● 20 g Agar (if making plates)
    ● Stir Plate & Bar (or a Stir Stick if not available)
    ● 1 L Beaker
    ● 1 L Distilled Water
    ● 1 L Graduated Cylinder
    ● 1 L Bottle
    ● 2 L Flask (if making plates)
    ● .01 g – 300 g capable Scale
    ● Weigh boats and spatulas
    ● Autoclave
    ● Optional: Autoclave tape and Pen
    Procedure
    1. Put a stir bar into a beaker and put on a stir plate
    a. If using a stir stick, just remember to stir the mixture with each new ingredient
    2. Pour approximately 600 mL of distilled water into the beaker
    3. Using a weighing boat, measure out 250 g of NaCl and pour it into the beaker.
    4. Turn the stir plate on low
    5. Repeat step 3 for the other substances except agar
    6. Turn the stir plate up a little higher and measure out approximately 200 mL of distilled water and
    pour it into the beaker
    7. After this has stirred for a while and it looks homogenous, pour the contents of the beaker into the
    graduated cylinder, holding another stir bar to the bottom of the beaker so that the stir bar will not
    fall out. Add enough distilled water to the cylinder to equal 1L
    8. Transfer to a bottle and prepare it to be autoclaved
    a. If making plates, add agar to the bottle that will be autoclaved

    Mustafa Umut Sarac
    Istanbul
    Last edited by Mustafa Umut Sarac; 09-20-2013 at 12:20 PM. Click to view previous post history.

  2. #2
    Mustafa Umut Sarac's Avatar
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    You can start to grow your halobacterium salinarium from salted codfish.

    9118 <-- CCM 2148 <-- I. Dundas 1.
    Accessioned in 1994.
    =CCM 2148. CCM Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic.
    Halobacterium salinarium.
    Category: Archaea.
    Medium: 168; Temperature: 37°C; Rehydration fluid: 307.
    Source: Salted codfish.
    Numerical taxonomy: [3282].
    Quinone: MK-8, MK-8(H2) [2070].
    DNA-DNA relatedness: [3366].
    Other taxonomic data: Polyamine [4558]; Sensitivity to antibiotics [3649]; Sensitivity of ribosome to antibiotics [3641,3699].
    Last edited by Mustafa Umut Sarac; 09-21-2013 at 03:22 AM. Click to view previous post history.

  3. #3
    Herzeleid's Avatar
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    Quite interesting, recently there were some photographic images produced using bacteria. May be they are similar to this method?

    How about sensitivity, exposure times, are there any visuals?
    Last edited by Herzeleid; 09-21-2013 at 06:42 AM. Click to view previous post history.

  4. #4
    Mustafa Umut Sarac's Avatar
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    Selam Serdar,

    I am surfing the internet for 15 years and I only found that an New York High School was experimenting to make these plates. I dont know how we would see an image from these plates but may be ultraviolet light reveals something. The plus side is the ability to record immense amount of information with that biology. Bacteriorhodopsin is the fastest working data recording and data processing medium also. Soviets makes radars to instantly track and identify the enemy with bacteriorhodopsin volumetric processors. Some say F22 have that kind of radar processors in their electronics . It is the best data recording medium in the world.

    Photography with that medium is the easiest thing you can do. And we can make electronic sensors with that medium also. Large sensors or linear array sensors.

    If picasso recorded an image on fungus at 1917 and we reveal it at 2013 , its strong and its a fast working ,camera speed medium also.

    I am thinking to follow molecular biology classes at istanbul from fatih university and you can learn more at bilkent.

    I am going to university next week to collect course notes of first year from class 2 students. May be I can find a professor to ask questions.

    Take care,

    Umut

  5. #5
    Mustafa Umut Sarac's Avatar
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    Its speed is same or faster than hologaphic plates. Volumetric holography with proteins would reveal lots of information on google.

  6. #6
    AgX
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    Quote Originally Posted by Herzeleid View Post
    Quite interesting, recently there were some photographic images produced suing bacteria.
    http://www.popsci.com/diy/article/2013-08/grow-photo

    http://www.ucsf.edu/news/2005/11/534...ng-photographs

    http://www.sciencemuseum.org.uk/antenna/livingfilm/

    http://www.nature.com/nature/journal...ture04405.html


    monochromatic-light induced breed of bacteria :
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053042/
    Last edited by AgX; 09-21-2013 at 06:07 AM. Click to view previous post history.

  7. #7
    Mustafa Umut Sarac's Avatar
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    Thank you AgX. These are different , may be forests take images of the sky upfront of them. That is taking images with living organism , its like photosynthesis of growth. Bacteriorhodopsin is dead and an protein.

  8. #8
    Herzeleid's Avatar
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  9. #9
    Mustafa Umut Sarac's Avatar
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    To put that protein in gelatin and coat a 35mm film and put in the camera an weak red laser light which will emit light when the shutter closing is a heartwarming technology. Scientists grow that solution in the coca cola bottles.

  10. #10
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    Image sensing and processing by a bacteriorhodopsin-based artificial photoreceptor
    Tsutomu Miyasaka and Koichi Koyama »View Author Affiliations

    Applied Optics, Vol. 32, Issue 31, pp. 6371-6379 (1993)
    http://dx.doi.org/10.1364/AO.32.006371

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    AbstractArticle InfoReferences (12)Cited ByMetrics
    Abstract
    Sensing and processing of optical information have been conducted with a unique bioelectronic image sensor that immobilizes bacteriorhodopsin (bR) as a photosensitive retinal protein. A thin film of bR-containing purple membranes was coated on a two-dimensional pixel array of electrodes and was made into a junction with an electrolyte gel layer having a counterelectrode to form an artificial photoreceptor. Photocurrent signals from each pixel showed a differential responsivity to light intensity, intrinsic to this liquid-junction photocell. Images detected and processed by the bR-based artificial photoreceptor were simultaneously displayed on a light-emitting-diode monitor panel through parallel signal-transmission circuitry. The experiment revealed that the photoreceptor is, as a retina model, capable of selectively detecting motion of images in real time and of performing vectorial extraction of their edge components, similar to the visual processing function of biological photoreceptors.

    © 1993 Optical Society of America

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