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  1. #1
    Mustafa Umut Sarac's Avatar
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    Bacteriorhodopsin Emulsion Recipe / Question for solgel step

    I found that bacteriorhodopsin emulsion recipe. Why solgel step is used , cant we use only 221 or
    something for 222 ?

    Its possible to record 5000 lp/mm with these emulsions.


    Bacteriorhodopsin (bR), polyvinyl alcohol (PVA), HCl, sodium borate, boric acid and
    gelatin (GE) were provided from Sigma.Triethanolamine (TEA) and tetraethoxysilane
    (TEOS) were provided from Fluka. The Glassy surface was provided from Sinagen.
    2.2. Preparation of bR films
    2.2.1. Preparation of gelatin-polyvinil alcohol matrix
    Bacteriorhodopsin films in polymer matrices were prepared by using the standard
    procedures [7,8]. One mg of bR was soaked in 1ml tri-distilled water for 20 min. Following,
    0.4 M of triethanolamine (TEA) aqueous solution was added to the bR suspension to give a
    TEA:bR molar ratio of 250:1 (which give maximal photosensitivity). 0.0005 gr of PVA and
    0.0005gr of GE powders were solved in 100ml tri-distilled water for 20 minutes to prepare
    PVA and GE solutions. The resulting solution was heated and stirred at 60 °C for 40 min.
    Finally, the appropriate ratio of bR-TEA and GE-PVA solutions were mixed and stirred for
    20–30 minutes to make a film-forming solution [7].
    2.2.2. Preparation of sol-gel matrix
    Tetraethoxysilane (TEOS) were used as precursors for the sol-gel glass. TEOS (7 ml), 3.0
    ml of distilled water and 0.1 ml of 0.04 M HC1 were mixed together and sonicated for 20
    min. The resulting product was diluted with an equal volume of distilled water. The resulting
    mixture (0.5 ml) was mixed with 0.25 ml sodium borate buffer solution (pH 9) and (0.1 ml,
    0.2 ml) bR solution to create a film-forming solution. Triethanolamine (TEA) was chosen as
    sensitizing additives. The concentration of chemical additives was in the range of 0.01–0.4
    M. For the preparation of chemically modified films, bR solution was mixed with TEA,
    before mixing with the sol mixture, and afterwards 0.02 ml of the bR-chemical additives
    mixture was added to 0.12 ml of the sol mixture [7,8].
    Last edited by Mustafa Umut Sarac; 10-05-2013 at 12:07 AM. Click to view previous post history.

  2. #2
    Mustafa Umut Sarac's Avatar
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    These films protects their records up to 7 years and can be deleted and reused. I learned step 222 uses TEOS and its not for amateurs.
    I am waiting any help for replacing step 222 with another step.
    Records change their colors visible and I dont have any idea how to print it. These emulsions used by hologram experts to record 100 times better area about fluid flow holography than ccd.

    I am hereby attaching the paper.
    Attached Files

  3. #3
    Mustafa Umut Sarac's Avatar
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    I think we dont need 222.

    I found that:

    Appl Biochem Biotechnol. 2012 Oct;168(4):936-46. doi: 10.1007/s12010-012-9832-3. Epub 2012 Sep 6.
    Extraction and purification of purple membrane for photochromic thin film development: application in photoelectrochemical investigation.
    Pandey PC, Pandey D, Singh R.
    Source
    Department of Applied Chemistry, Indian Institute of Technology, Banaras Hindu University, Varanasi 221005, India. pcpandey.apc@itbhu.ac.in
    Abstract
    Purple membrane (PM) has been extracted and purified from archaebacteria for thin film development. The purified purple membrane is isolated in 1 % polyvinyl alcohol solution for making thin film within gelatin and organically modified silicate matrices. For thin film within gelatin matrix, homogenized purple membrane suspension is mixed with 8 % gelatin and poured into a specially designed block with desired thickness of spacer having hydrophobicity followed by gelatinization of the same over home-made thermostatic control unit at 38 °C. The gelatinized matrix is then allowed to dry under controlled conditions of humidity and temperature. The films of varying thicknesses ranging between 40, 50, and 60 μ are used for photo-electrochemical measurements. The results on photo-electrochemistry of non-oriented purple membrane film provides valuable information on the generation of forward (light on) and backward (light off) photocurrent as a function of: (a) applied potential and (b) film thickness. An increase in applied negative potential increases the amplitude of photocurrent whereas decrease in film thickness facilitates the reversibility of photocurrent response.
    PMID: 22956275 [PubMed - indexed for MEDLINE]



 

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