HP5+/D3200 Exposure Under the Microscope

[VaryaV]

It wasn't the exposures I was struggling with, it was the on/off camera lever. I pretty much know what my shutter speeds will be as there is no lens on the camera itself. It's the lighting adjustments and focus that would be great to have test shots of. As you know the DOF in micro is pretty 2-dimensional you have to create the illusion of depth with the lighting. The focus is so tiny but you have so many creative options where you want that focus to be and bokeh takes care of the rest. What's great about scope lamps is that the lighting is pin point and adjustable, that takes the place of f-stop in a way. If you guys ever need pin point lighting that is definitely the way to go, and fiber optics too have a really soft look to them and they can get almost into anything.

[hpulley]

The Fuji stuff I use won't fit the SX-70 though The Impossible Project film might, perhaps the Fuji Instax stuff, I'm not sure.

[tkamiya]

I'm NOT sure if this work with film but in digital microscope photography world, folks are doing what is called image stacking. As you already know, DOF of microscope is very tiny. Even with blood cells (which are about 100th of human hair), you could focus on various points of the cell. To counter act this, there is a technique where you'd focus on top of the subject, snap. Move the focus point, snap. Move some more, snap.... all the way to the bottom of the subject. Then, combine these all images into one.

I wonder if you can do this with film using multiple exposure? Exposure setting and keeping everything steady would be a challenge, though....
Just a thought.....

[VaryaV]

I'm NOT sure if this work with film but in digital microscope photography world, folks are doing what is called image stacking. As you already know, DOF of microscope is very tiny. Even with blood cells (which are about 100th of human hair), you could focus on various points of the cell. To counter act this, there is a technique where you'd focus on top of the subject, snap. Move the focus point, snap. Move some more, snap.... all the way to the bottom of the subject. Then, combine these all images into one.

I wonder if you can do this with film using multiple exposure? Exposure setting and keeping everything steady would be a challenge, though....
Just a thought.....

oh, that certainly sounds exciting and worth a try. I would think because your DOF is so minute you could stack images without visual overlap... I'm thinking especially of that juicy bokeh hidden in the crevasses that would be so stunning. Think of the visual potential... thanks so much for the suggestion. Very cool!

[pgomena]

If you own a Gossen Luna Pro SBC or other model with a small diffusion cap over the sensor, just put the dome into the tube and take a reading. This actually works.

Peter Gomena