Tygon tubing must be selected according to the size of the head. The tubing is numbered to fit every size head unless you have a universal type head.
Go here to select: http://www.coleparmer.com/catalog/pr...x.asp?cls=8071
Go here to order: http://www.espchemicals.com/about.cfm?&DID=19 and speak to Phil.
If you get the wrong size tubing for a non-universal head, it will not work.
You must calibrate the pump/tubing combination first to turn rpms/minute into ml/minute.
And yes, it will be in the book and the DVD. That is part of what delayed the book. My friends suggested adding that chapter to the book and DVD. I did the DVD portion first and then the book which is in revision #5 and here on my stack of work being done.