Quote Originally Posted by BetterSense View Post
Recently I have been trying to photograph a small round seed pod at about 1:1 with my Olympus OM1 and 50mm Macro with extension rings. I put the lens on f/22 which is probably an actual f/45 or so at 1:1. The 8x10 proofs I made aren't impressively sharp, and I think I have eliminated camera/subject motion as a cause.
Effective Aperture=Nominal Aperture x (magnification +1)

So, in your case (above) EA=22(1+1)=44